This also suggests that there could be some other properties of WSSV ie. 1 promoter- mediated baculovirus vaccine could present better immunogenicity without biosafety concerns in vaccine preparation. In addition, compared with inactivated H5 influenza virus vaccines at the same dose of HA protein (p < 0. This allows it to induce higher level of specific antibody response in immunized poultries at the same number of baculovirus copies. 1 promoter supports the abundant production of HA in baculovirus system, as compared with other promoters tested here. Although baculovirus- expressed hemagglutinin influenza vaccines have been widely used and well characterized in different ways and under variant vector designs. Therefore, for intramuscular injections, most of HA expression was detected in chicken thymus. Also, as the major organ involved in immunity of avian species, thymus supports exogenous antigen expression. For intranasal inoculation, lung is the major organ to directly contact immunogen upon vaccination, where VSV G mediates virus entry and HA expresses with its individual promoter. The results were repeatedly observed in most chickens from each group and considered to be significant. As the direct evidence for gene transduction, HA antigen expression in tissues was revealed in IHC assays. N1 influenza virus, dosage was based on HA contents (6 ug) due to differences in viral property. Meanwhile, when the comparison was performed between baculovirus and attenuated H5. As shown here, at the same dosage of baculovirus, constructs with WSSV ie.ĬMV promoter, confirming the higher HA expression level by WSSV ie. In the comparison of different promoters in the same type of baculovirus construct, vaccine dose was based on virus copies rather than protein contents in order to differentiate the HA production efficiency by different promoters with the same amount of baculovirus copies (1. HA- specific immune response in chickens. HA expression on viral surface with WSSV ie.
For gene transduction, although CMV promoter is stronger in chicken cells than WSSV ie. HA expressed in baculovirus served as exogenous antigen to stimulate primary immune response, while HA de novo expression in chicken tissue will contribute to the trigger of new HA- antibody production for further protection in a long run. The results confirmed the role of WSSV ie. The TriStar2 now also supports time resolved fluorescence energy transfer. Sustained transgene expression in cartilage defects in vivo after transplantation of articular chondrocytes modified by lipid. Despite a good understanding of all these attractive features of baculovirus, gene therapy. The complexes can be used for producing pharmaceutical compounds for the transfer of therapeutically active genes in mammalian cells. However, CMV promoter might be relatively weak for protein expression in insect cells. In this study, CMV promoter was used for major comparison because it displayed activity in both mammalian and insect cells as indicated in luciferase assays and it is widely used for protein expression and gene transduction in numerous cell lines. HA production and gene transduction, relying on its promoter efficiency in both vertebrate and invertebrate species. The recombinant baculoviruses under WSSV ie. 1 promoter is more efficient than CMV promoter to express H5 hemagglutinin from influenza virus in baculovirus as a chicken vaccine. An Efficient System for Cap- and Poly(A)-Dependent Translation In Vitro. Imaging of transduced cells and tissues is. Imaging of transduced cells and tissues is valuable in developing gene transfer. Herm - high energy radio monitor lb 500, luminometer, mithras lb 940.
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